|
|
|
|
| HTP equipment |
|
Product |
Pack of
|
|
Specification |
REF |
 |
NucleoVac 96
Vacuum Manifold |
1
|
|
vacuum manifold, consists of manifold base and lid, a spacer set, and a waste container set, for use of NucleoSpin
8-well strips on the NucleoVac 96 the Starter Set A is required (see below) |
740681 |
 |
NucleoVac
Vacuum Regulator |
1
|
|
for controlling of vacuum |
740641 |
|
|
|
|
|
|
 |
NucleoSpin® Dummy Strips |
6 strips
|
|
for closing unused rows of Column Holders A and B during vacuum processing of NucleoSpin® 8-well kits |
740685 |
 |
MN Frame |
1
|
|
for optimized handling of 96-well plates with vacuum manifold on BioRobot® 9600, 9604, and 3000 (Qiagen), MultiPROBE® II / Janus (PerkinElmer), Biomek® 2000 / 3000 and FX / NX (Beckman Coulter) |
740680 |
 |
NucleoMag® SEP |
1
|
|
magnetic separator, for use with 96-well plates (e.g., REF 740481) |
744900 |
 |
Rubber Pad |
2
|
|
reusable mat to cover unused rows of a 96-well plate |
740640 |
Column Holder A |
Starter Set A
|
1 set
|
|
for processing NucleoSpin® 8-well strips under vacuum on NucleoVac 96 Vacuum Manifold or similar manifolds, contains
2 Column Holders A, NucleoSpin® Dummy Strips |
740682 |
|
Column Holder B
Column Holder D
|
Starter Set B |
1 set
|
|
for processing NucleoSpin® 8-well strips on the Qiagen BioRobot® 9600 / 9604 / 3000, contains
1 Column Holder B, 1 Column Holder D, NucleoSpin® Dummy Strips |
740683 |
Column Holder C |
Starter Set C |
1 set
|
|
for processing NucleoSpin® 8-well strips under centrifugation, contains 2 Column Holders C, MN Square-well Blocks, Rack of Tube Strips |
740684 |
|
| |
|
|
| High throughput (HTP) consumables |
|
Product |
Pack of
|
|
Specification |
REF |
 |
MN Wash Plate |
4
24
|
|
to facilitate drying of the DNA / RNA binding module and to minimize the risk of cross-contamination
as above |
740479
740479.24 |
 |
The silica-membrane technology based NucleoSpin® kits contain the unique MN Wash Plate to prevent the bottom of the binding module from being contaminated by ethanolic wash buffers during the washing steps, thus improving the drying of the binding module under vacuum and preventing EtOH contamination of the eluted DNA. The funnel shape of each well allows easy positioning of the binding plate, manually or automated. |
|
 |
Square-well Block |
4
24
|
|
for use with NucleoMag® SEP (744900)
as above |
740481
740481.24
|
 |
MN Square-well Block |
4
24
|
|
96-well blocks with 2.1 mL square wells
as above |
740476
740476.24 |
|
Culture Plate |
4 sets
24 sets
|
|
Square-well Block with 2.1 mL square wells used for growing, harvesting, or lysing of bacterial cultures, with Gas-permeable Foil
as above |
740488
740488.24 |
 |
Round-well Block
Round-well Block
with Cap Strips |
20
4 sets
24 sets
|
|
96-well blocks with 1.2 mL round wells used for e.g., mixing steps and collecting elution fractions
set consists of 1 Round-well Block and 12 Cap Strips
as above |
740671
740475
740475.24 |
 |
Round-well Block Low |
4
4 sets
24 sets
|
|
96-well blocks with 0.8 mL round wells
96-well blocks with 0.8 mL round wells, and Self-adhering Foil
as above |
740482
740487
740487.24 |
 |
Elution Plate
U-bottom |
24
|
|
96-well microplates with 300 µL u-bottom, with Self-adhering Foil |
740486.24 |
 |
Rack of Tube Strips |
5 sets
4 sets
24 sets
|
|
1 set consists of 1 rack, 12 strips with 8 tubes each
1 set consists of 1 rack, 12 strips with 8 tubes each, and 12 cap strips
as above |
740637
740477
740477.24 |
 |
Cap Strips |
48
288
|
|
for sealing of Tube Strips, Round-well Blocks
as above
|
740478
740478.24 |
 |
Gas-permeable Foil |
50
|
|
to cover Square-well blocks during incubation of bacterial cultures |
740675 |
 |
Self-adhering PR Foil |
50
|
|
adhesive tape foils for sealing 96-well plates for air-tight storage of DNA / RNA |
740676 |
 |
NucleoSpin® Plasmid Filter Strips |
48
|
|
for clarification of lysates,
for use under vacuum or centrifugation |
740730.48F |
 |
NucleoSpin® RNA Filter Strips |
12
60
|
|
for filtration of cell and tissue homogenates, for use under cavuum or centrifugation
as above
|
740699.12F
740699.60F
|
 |
NucleoSpin® RNA
Filter Plate |
4
|
|
for filtration of cell and tissue homogenates,
for use under vacuum or centrifugation |
740711 |
|
NucleoSpin® Trace
Filter Plate |
20
|
|
for lysis of samples and subsequent removal of particulate matter, for use under vacuum or centrifugation |
740677 |
|
Receiver Plates
20 µm |
4
|
|
96-well plates with inserted filter frits of 20 µm pore size, suitable for centrifugation and vacuum |
740686.4 |
Receiver Plates
20 µm hydrophilized |
4
|
|
96-well plates with inserted hydrophilized filter frits of 20 µm pore size, suitable for gravity flow, centrifugation, and vacuum |
740687.4 |
|
Receiver Plates
50 µm |
4
|
|
96-well plates with inserted filter frits of 50 µm pore size, suitable for centrifugation and vacuum |
740688.4 |
Receiver Plates
50 µm hydrophilized |
4
|
|
96-well plates with inserted hydrophilized filter frits of 50 µm pore size, suitable for gravity flow, centrifugation, and vacuum |
740689.4 |
 |
KingFisher® 96
Accessory Kit A
|
1 set
|
|
Square-well Blocks, Deep-well Tip Combs,
Elution Plates, for 4 x 96 NucleoMag® 96 Tissue / Trace / Virus preps using KingFisher® 96 platform |
744950 |
|
KingFisher® 96
Accessory Kit B
|
1 set
|
|
Square-well Blocks, Deep-well Tip Combs,
Elution Plates, for 4 x 96 NucleoMag® Blood 200 µL and 96 NucleoMag® 96 Plant / RNA preps using KingFisher® 96 platform |
744951 |
|
| |
|
|
| Single prep consumables |
|
Product |
Pack of
|
|
Specification |
REF |
| NucleoBond® consumables |
 |
NucleoBond® Folded Filters
|
50
|
|
for use with NucleoBond® AX 100 Columns |
740561 |
|
NucleoBond® Folded
Filters XL
|
50
|
|
for use with NucleoBond® AX 500 and
AX 2000 Columns |
740577 |
 |
NucleoBond® Rack
Small
|
1
|
|
for use with NucleoBond® AX 20 Columns |
740562 |
 |
NucleoBond® Rack Large
|
1
|
|
for use with NucleoBond® AX 100, AX 500, AX 2000, AX 10000, BAC 100, and Xtra Midi Columns |
740563 |
 |
NucleoBond® Xtra Combi Rack
|
1
|
|
for use with NucleoBond® Xtra Midi, Maxi,
AX 100, AX 500, AX 2000, and 10000 Columns |
740415 |
| NucleoSpin® consumables |
 |
Collection Tubes
(2 mL) |
1000
|
|
|
740600 |
 |
NucleoSpin® Filters
|
50
|
|
for filtration of cell and tissue homogenates |
740606 |
 |
NucleoSpin® Funnel Column
|
30 sets
|
|
set of NucleoSpin® Funnel Column, Collection
Tube (50 mL), and Collection Tube (0.5 mL) |
740959 |
| Protino consumables |
 |
Protino® Columns
14 mL
|
10
|
|
empty columns for use with Protino®
Ni-TED/IDA Resin, Protino® Ni-NTA Agarose and Protino® Glutathione Agarose 4B |
745250.10 |
|
Protino® Columns
35 mL
|
10
|
|
as above |
745255.10 |
| Other consumables |
 |
bondEx Folded Filters XL |
50
|
|
|
740705 |
 |
Receiver Columns
20 µm |
10
|
|
mini spin columns with inserted hydrophobic frit of 20 µm pore size,
to be used for general filtration purposes as well as for retaining chromatographic resins. The columns are delivered with capped outlets, inserted into Collection Tubes (2 mL) |
740522.10 |
|
|
50
|
|
as above |
740522.50 |
|
|
250
|
|
as above |
740522.250 |
|
| |
|
|
| Buffers and enzymes |
|
Product |
Pack of
|
|
Specification |
REF |
|
Plasmid DNA purification, NucleoBond® |
|
Buffer ELU |
600 mL
|
|
elution buffer |
740316.600 |
|
Buffer EQU |
1000 mL
|
|
equilibration buffer |
740317.1000 |
|
Buffer LYS |
1000 mL
|
|
lysis buffer |
740329.1000 |
|
Buffer N2 |
500 mL
|
|
equilibration buffer |
740527.1 |
|
Buffer N3 |
1000 mL
|
|
wash buffer |
740528.1 |
|
Buffer N5 |
500 mL
|
|
elution buffer |
740529.1 |
|
Buffer NEU |
1000 mL
|
|
neutralization buffer |
740348.1000 |
|
Buffer RES |
1000 mL
|
|
resuspension buffer, without RNase A |
740363.1000 |
|
Buffer S1 |
500 mL
|
|
resuspension buffer, without RNase A |
740516.1 |
|
Buffer S2 |
500 mL
|
|
lysis buffer |
740517.1 |
|
Buffer S3 |
500 mL
|
|
neutralization buffer |
740518.1 |
|
Buffer WASH |
1000 mL
|
|
wash buffer |
740375.1000 |
|
NucleoBond® Buffer Set I |
1 set
|
|
for isolation of low-copy plasmids, cosmids, BACs, PACs, and P1 constructs,
only applicable with NucleoBond® PC kits, sufficient for 10 AX 500 columns (Maxi preps), 20 AX 100 columns (Midi preps). Set incl. RNase A
|
740601 |
|
NucleoBond® Buffer Set III |
1 set
|
|
for isolation of genomic DNA from bacteria and yeast,
only applicable with NucleoBond® AXG columns,
set incl. RNase A |
740603 |
|
NucleoBond® Buffer Set IV |
1 set
|
|
for isolation of genomic DNA from tissue,
only applicable with NucleoBond® AXG columns,
set incl. Proteinase K, RNase A |
740604 |
|
NucleoBond® Xtra Buffer Set I |
1 set
|
|
for isolation of low-copy plasmids, cosmids, BACs, PACs, and P1 constructs,
only applicable with NucleoBond® Xtra kits, sufficient for 10 Xtra Maxi preps or 20 Xtra Midi preps. Set includes buffers RES, LYS, NEU, and RNase A |
740417 |
|
NucleoBond® Xtra EF Buffer Set I |
1 set
|
|
for isolation of endotoxin-free low-copy plasmids, cosmids, BACs, PACs, and P1 constructs,
only applicable with NucleoBond® Xtra EF kits, sufficient for 10 Xtra EF Maxi preps or 20 Xtra EF Midi preps. Set includes buffers RES-EF, LYS-EF, NEU-EF, and RNase A |
740427 |
|
Plasmid DNA purification, NucleoSpin® |
|
Buffer A1 |
1000 mL
|
|
resuspension buffer, without RNase A |
740911.1 |
|
Buffer A2 |
1000 mL
|
|
lysis buffer |
740912.1 |
|
Buffer A3 |
1000 mL
|
|
neutralization buffer |
740913.1 |
|
Buffer A4 Concentrate |
20 mL
|
|
wash buffer concentrate, for 100 mL Buffer A4 |
740914 |
|
Buffer A4 Concentrate |
200 mL
|
|
as above, for 1000 mL Buffer A4 |
740914.1 |
|
Buffer AE |
1000 mL
|
|
elution buffer |
740917.1 |
|
Buffer AW |
1000 mL
|
|
wash buffer |
740916.1 |
|
NucleoSpin® Plasmid Buffer Set |
1 set
|
|
for isolation of low-copy plasmids,
only applicable with NucleoSpin® Plasmid kits, buffers A1, A2, A3, RNase A, suitable for 300 preps |
740953 |
|
DNA clean-up |
|
Buffer NT |
100 mL
|
|
binding buffer |
740614.100 |
|
Buffer NT1 |
100 mL
|
|
binding buffer |
740596.100 |
|
Buffer NT2 |
100 mL
|
|
binding / wash buffer |
740597 |
|
Buffer NT3 Concentrate |
20 mL
|
|
wash buffer concentrate,
for 100 mL Buffer NT3 |
740598 |
|
Buffer NTB |
150 mL
|
|
binding buffer,
for clean-up of SDS-containing samples |
740595.150 |
|
Buffer NTB |
1000 mL
|
|
binding buffer |
740595.1 |
|
Buffer NTC |
100 mL
|
|
binding buffer,
for clean-up of single-stranded DNA |
740654.100 |
|
Total RNA isolation |
|
Buffer RA1 |
50 mL
|
|
lysis buffer |
740961 |
|
Buffer RA1 |
500 mL
|
|
as above |
740961.500 |
|
Buffer RAP |
50 mL
|
|
lysis buffer |
740936.50 |
|
Buffer RAP |
500 mL
|
|
as above |
740936.500 |
|
Buffer RL1 |
50 mL
|
|
lysis buffer |
740385.50 |
|
Buffer RL1 |
125 mL
|
|
as above |
740385.125 |
|
Buffer RP1 |
50 mL
|
|
lysis buffer |
740934.50 |
|
Buffer RP1 |
500 mL
|
|
as above |
740934.500 |
|
Protein Solving Buffer Set
PSB/TCEP |
1 set
|
|
7.5 mL Buffer PSB and 107 mg TCEP (Reducing Agent),
applicable with NucleoSpin® RNA/Protein, NucleoSpin® TriPrep, and NucleoSpin® miRNA kits |
740941 |
|
Genomic DNA isolation |
|
Buffer B3 |
100 mL
|
|
lysis buffer |
740920 |
|
Buffer B5 Concentrate |
20 mL
|
|
wash buffer concentrate, for 100 mL Buffer B5 |
740921 |
|
Buffer BE |
100 mL
|
|
elution buffer |
740306.100 |
|
Buffer BQ1 |
125 mL
|
|
lysis buffer |
740923 |
|
Buffer BW |
100 mL
|
|
wash buffer |
740922 |
|
Buffer C1 |
100 mL
|
|
lysis buffer |
740930 |
|
Buffer Set C2/C3 |
1 set
|
|
binding buffer, for 125 mL Buffer C4 |
740935 |
|
Buffer CF |
1000 mL
|
|
lysis buffer |
740946 |
|
Buffer CW |
100 mL
|
|
wash buffer |
740932 |
|
Buffer FLB |
500 mL
|
|
lysis buffer |
740322.500 |
|
Buffer PC |
125 mL
|
|
binding buffer |
740937 |
|
Buffer PL1 |
125 mL
|
|
lysis buffer |
740918 |
|
Buffer Set PL2/PL3 |
1 set
|
|
lysis buffers,
100 mL Buffer PL2 and 25 mL Buffer PL3 |
740919 |
|
Buffer PW1 |
125 mL
|
|
wash buffer |
740938 |
|
Buffer PW2 Concentrate |
50 mL
|
|
wash buffer concentrate,
for 250 mL Buffer PW2 |
740939 |
|
Buffer T1 |
25 mL
|
|
lysis buffer |
740940.25 |
|
NucleoSpin® DNA Trace Bone
Buffer Set |
1
|
|
for isolation of DNA from bones,
only applicable with NucleoSpin® DNA Trace kits, sufficient for 25 preparations |
740943.25 |
|
Enzymes |
|
Proteinase K |
100 mg
|
|
|
740506 |
|
RNase A |
50 mg
|
|
|
740505.50 |
|
RNase A |
100 mg
|
|
|
740505 |
|
rDNase Set
click for detailed information |
1 set
|
|
recombinant DNase and Reaction Buffer for rDNase,
for 50 minipreparations of total RNA |
740963 |
|
| |
|
|
| Protein quantification |
|
Product |
Assays
|
|
Specification |
REF |
|
Protein Quantification Assay |
50
|
|
Buffer PSB, Quantification Reagent, BSA |
740967.50 |
|
|
250
|
|
as above |
740967.250 |
|
| |
|
|
 |